Zeitschriftenaufsatz | 2026 Open Access

Somatic STAT5BN642H mutations shape variable immune landscapes resulting in heterogenous immune diseases.

Autor:in
Grün, S.; Rensing-Ehl, A.; Suske, T.; Wolter-Mess, J.; Gehrig, J.; Mann, J.; König, C.; Hauri, M.; Heeg, M.; Leahy, T. R.; Genevieve, D.; Gaillard, J. B.; Broly, M.; Bourdin, A.; Castro, C.; Seidel, L.; Bengsch, B.; Aichele, P.; Weißert, K.; Fernandez-Orth, J.; Schipperges, V.; Andrieux, G.; Boerries, M.; Cabezas-Wallscheid, N.; Erlacher, M.; Elling, R.; Speckmann, C.; Heller, L.; Schulte, B.; Lorenz, M.; Schwarz, K.; Moriggl, R.; Ehl, S.
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Suske, Tobias
Journal
Journal of Allergy and Clinical Immunology
Schlagwörter
Atopy; Autoimmunity; Clonal hematopoiesis; Hematopoietic stem cells; Immune dysregulation; STAT5B; Somatic mosaicism; T cell lymphoproliferation; mTOR inhibition
Dokumenten­typ
Originalarbeit
CC Lizenz
CC-BY
Open Access Type
Hybrid
ISSN/eISSN
0091-6749 - 1097-6825
DOI
Öffnen URL 10.1016/j.jaci.2025.12.993
PubMed ID
Öffnen URL 41453450

Weitere Details

Inborn errors of immunity (IEI) are traditionally understood as monogenic germline disorders. However, somatic mosaicism can also result in immune-mediated diseases, mimicking IEI. While early postzygotic mosaicism is the predominant mechanism, genetic variants causing a selective advantage to hematopoietic progenitors and/or mature immune cells may cause immune dysregulation and initiate disease at any age. Somatic mosaicism for STAT5BN642H was linked to severe allergic disease in infancy but its full clinical spectrum and underlying mechanisms remain incompletely defined. To elucidate how somatic N642H mutations of the STAT5B gene shape lineage-specific mosaicism, immune cell function and clinical phenotypes. We investigated three new patients - including one adult - with STAT5BN642H mosaicism using deep sequencing, flow and mass cytometry, and functional immune assays. Mutant cell distribution was mapped across blood lineages. A mouse model with mosaic STAT5BN642H mutation in hematopoietic stem cells was generated to study clonal dynamics and immune phenotypes. Patients displayed variable lineage mosaicism correlating with two predominant clinical outcomes: early-onset severe atopy with hypereosinophilia, and autoimmune-lymphoproliferative immunodeficiency with expansions of CD8 and γδ T cells. Functional studies revealed enhanced IL-2-mediated proliferation, effector differentiation, and oligoclonal T cell expansions. In mice, a few mutant HSCs reproduced the patient lineage-skewed immune landscapes with variable growth advantage of mutant cells across hematopoietic development and recapitulated patient T cell phenotypes. Targeted mTOR inhibition successfully controlled lymphoproliferation in patients. Our findings illuminate how a single somatic variant in few stem cells can remodel hematopoiesis, generating variable immune mosaics and heterogeneous immune disease. Copyright © 2025. Published by Elsevier Inc.

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