Zeitschriftenaufsatz | 2026 Open Access

Structure and Function of a Multi-Megadalton Virus-Like Proteolytic Dodecahedron

Autor:in

Madej, Mariusz; Rodriguez-Banqueri, Arturo; Mizgalska, Danuta; Szmigielski, Borys; Nowakowska, Zuzanna; Benedyk-Machaczka, Malgorzata; Bzowska, Monika; Mikruta, Katarzyna; Ramirez-Larrota, Juan Sebastian; Agunanne, Chinanu; Julien, Olivier; O'Donoghue, Anthony J.; Scavenius, Carsten; Lopez, Mario; Marcos, Enrique; Koziej, Lukasz; Glatt, Sebastian; Guerra, Pablo Contreras; Eckhard, Ulrich; Potempa, Jan; Gomis-Ruth, F. Xavier

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Glatt, Sebastian

Journal

Angewandte Chemie-International Edition

Schlagwörter

bacterial virulence; cryoelectron microscopy; dodecahedron; icosahedral symmetry; multimeric metallopeptidase; oral microbiome dysbiosis; periodontal disease; x-ray crystallography; zymogen activation

Dokumententyp

Originalarbeit

CC Lizenz

CC-BY

Open Access Type

Hybrid

ISSN/eISSN

1433-7851 - 1521-3773

DOI

10.1002/anie.5597279

WoS ID

WOS:001872244800001

PubMed ID

SDG Hauptzuordnung

03 … Gesundheit und Wohlergehen

Weitere Details

Seitenanzahl

14

Natural pentamer dodecahedra (Ddhs) span six orders of magnitude in diameter. Among proteins, only two catalytic Ddhs have been structurally characterized: lumazine synthase (LS) and the core of pyruvate dehydrogenase (PDH). Zuzalysin (ZUZ) is a approximate to 95-kDa metallopeptidase secreted for virulence by Porphyromonas gingivalis. Calcium converts latent flexible monomers into active approximate to 0.5-MDa pentamers that further assemble hierarchically into bipentamers, tripentamers, and a approximate to 5.6-MDa, approximate to 355-& Aring; virus-like dodecahedron (DdhZUZ). Experimental structures (1.8-3.6 & Aring;) across these states reveal the molecular basis of activation, association, and catalysis, culminating in DdhZUZ, which is physiologic, exceeds small viral capsids, and has 20 main entry pores and 60 lumen-facing active sites. ZUZ represents the largest catalytic protein assembly resolved at high resolution, exceeding LS, PDH, and major peptidase complexes in size and/or resolution.

Jagiellonian University

University of Louisville

University of Alberta

University of California San Diego

Institut de Biologia Molecular de Barcelona

Aarhus University

Universitat de Barcelona

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Natural pentamer dodecahedra (Ddhs) span six orders of magnitude in diameter. Among proteins, only two catalytic Ddhs have been structurally characterized: lumazine synthase (LS) and the core of pyruvate dehydrogenase (PDH). Zuzalysin (ZUZ) is a approximate to 95-kDa metallopeptidase secreted for virulence by Porphyromonas gingivalis. Calcium converts latent flexible monomers into active approximate to 0.5-MDa pentamers that further assemble hierarchically into bipentamers, tripentamers, and a approximate to 5.6-MDa, approximate to 355-& Aring; virus-like dodecahedron (DdhZUZ). Experimental structures (1.8-3.6 & Aring;) across these states reveal the molecular basis of activation, association, and catalysis, culminating in DdhZUZ, which is physiologic, exceeds small viral capsids, and has 20 main entry pores and 60 lumen-facing active sites. ZUZ represents the largest catalytic protein assembly resolved at high resolution, exceeding LS, PDH, and major peptidase complexes in size and/or resolution.

Jagiellonian University

University of Louisville

University of Alberta

University of California San Diego

Institut de Biologia Molecular de Barcelona

Aarhus University

Universitat de Barcelona

Download
RDF/XML-Format
JSON-LD-Format
Turtle-Format
N3-Format