Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

Autor:in

Cacciotti, Giulia; Caputo, Beniamino; Selvaggi, Carla; La Sala, A.; Vitiello, Laura; Diallo, Diawo; Ceianu, Cornelia-Svetlana; Antonelli, Guido; Nowotny, Norbert; SCAGNOLARI, CAROLINA

Abstrakt

Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV 12 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2. (C) 2015 Elsevier Inc. All rights reserved.

Dokumententyp

Originalarbeit

DOI

10.1016/j.virol.2015.07.015

PubMed ID

MEDLINE:26280469