Zeitschriftenaufsatz
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2015
Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Autor:in
Jambor, Helena Klara; Surendranath, Vineeth; Kalinka, Alex; Mejstrik, Pavel; Saalfeld, Stephan; Tomancak, Pavel
Journal
Abstrakt
mRNA localization is critical for eukaryotic cells and affects numerous transcripts, yet how cells regulate distribution of many mRNAs to their subcellular destinations is still unknown. We combined transcriptomics and systematic imaging to determine the tissue-specific expression and subcellular distribution of 5862 mRNAs during Drosophila oogenesis. mRNA localization is widespread in the ovary and detectable in all of its cell types-the somatic epithelial, the nurse cells, and the oocyte. Genes defined by a common RNA localization share distinct gene features and differ in expression level, 3'UTR length and sequence conservation from unlocalized mRNAs. Comparison of mRNA localizations in different contexts revealed that localization of individual mRNAs changes over time in the oocyte and between ovarian and embryonic cell types. This genome scale image-based resource (Dresden Ovary Table, DOT, http://tomancak-srv1.mpi-cbg.de/DOT/main.html) enables the transition from mechanistic dissection of singular mRNA localization events towards global understanding of how mRNAs transcribed in the nucleus distribute in cells.
Schlagwörter
Animals; Cell Nucleus/metabolism; Drosophila melanogaster/genetics*; Drosophila melanogaster/growth & development*; Female; Gene Expression Regulation, Developmental; Imaging, Three-Dimensional*; In Situ Hybridization, Fluorescence; Oogenesis/genetics; Ovary/metabolism; RNA Transport/genetics*; RNA, Messenger/genetics; RNA, Messenger/metabolism; Time Factors;
Dokumententyp
Originalarbeit
CC Lizenz
CCBY
Open Access Type
Gold
ISSN/eISSN
2050-084X -
10.7554/eLife.05003
WoS ID
PubMed ID