Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi.

Autor:in

Hekmat, Omid; Kim, Young-Wan; Williams, Spencer J; He, Shouming; Withers, Stephen G;

Schlagwörter

Amino Acid Sequence; Binding Sites; Biotinylation; Carbohydrate Sequence; Carbohydrates/chemistry; Catalytic Domain; Cellulomonas/enzymology*; Chromatography, Liquid; Cloning, Molecular; Disaccharides/antagonists & inhibitors; Glycoside Hydrolases/chemistry*; Hydrogen-Ion Concentration; Kinetics; Mass Spectrometry; Models, Chemical; Molecular Sequence Data; Peptides/chemistry*; Polymerase Chain Reaction; Protein Structure, Tertiary; Proteins/chemistry; Proteome/chemistry; Proteomics/methods; Sequence Homology, Amino Acid; Spectrometry, Mass, Electrospray Ionization; Streptomyces/metabolism; Temperature; Time Factors;

ISSN/eISSN

0021-9258 -

WoS ID

WOS:000232561200009