Novosphingobium pokkalii sp nov, a novel rhizosphere-associated bacterium with plant beneficial properties isolated from saline-tolerant pokkali rice

Autor:in

Krishnan, Ramya; Menon, Rahul R.; Likhitha; Busse, Hans-Juergen; Tanaka, Naoto; Krishnamoorthy, Srinivasan; Natarajan, Ramesh Kumar

Abstrakt

Pokkali rice varieties are known for their saline tolerance when specifically grown in coastal saline affected agri-fields of southern Kerala. These fields are prone to seawater intrusion. During characterization of phytobeneficial rhizobacteria from this pokkali rice, L3E4(T) was isolated. This strain showed some plant growth-promoting functions (production of indole acetic acid (IAA), acetoin, and siderophore), biofilm formation and capacity to use a wide range of plant -derived organic compounds. In planta assay under axenic conditions showed a positive effect of L3E4(T) on pokkali rice growth; importantly, it was able to attach and colonize pokkali rice roots in the presence of natural seawater, a key adaptation required for survival in pokkali rice fields. Phylogenetic analysis using 16S rRNA, recA, and gyrB gene sequences showed that strain L3E4(T) belongs to the genus Novosphingobium, with Novosphingobium capsulatum GIFU 11526(T) and Novosphingobium rhizosphaerae JM.1(T) being the nearest phylogenetic relatives. In addition, DNA-DNA hybridization analysis and phenotypic traits established that this strain belongs to a novel Novosphingobium species, for which we propose the name Novosphingobium pokkalii sp. nov. The type strain is represented by strain L3E4(T) (=MTCC 12357(T) = KCTC 42224(T)). (C) 2016 Institut Pasteur.

Dokumententyp

Originalarbeit

DOI

10.1016/j.resmic.2016.09.001

PubMed ID

MEDLINE:27639667