Zeitschriftenaufsatz
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2015
Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation
Autor:in
Schlötterer, Christian; Kofler, Robert; Versace, Elisabetta; Tobler, Ray; Franssen, S. U.
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Journal
Abstrakt
Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.
Schlagwörter
Adaptation, Physiological/genetics*; Animals; Biological Evolution*; Drosophila melanogaster/genetics; Gene Frequency; Genetic Association Studies; Genetic Variation*; Genotype; High-Throughput Nucleotide Sequencing*; Phenotype; Polymorphism, Single Nucleotide; Research Design;
Dokumententyp
Übersichtsarbeit
CC Lizenz
CCBYNCSA
Open Access Type
Hybrid
ISSN/eISSN
0018-067X - 1365-2540
10.1038/hdy.2014.86
WoS ID
PubMed ID