Rational engineering of mannosyl binding in the distal glycone subsites of Cellulomonas fimi endo-beta-1,4-mannanase: mannosyl binding promoted at subsite -2 and demoted at subsite -3.

Autor:in

Hekmat, Omid; Lo Leggio, Leila; Rosengren, Anna; Kamarauskaite, Jurate; Kolenova, Katarina; Stålbrand, Henrik;

Schlagwörter

Amino Acid Substitution/genetics; Binding Sites/genetics; Carbohydrate Sequence; Cellulomonas/enzymology*; Cellulomonas/genetics; Cellulomonas/metabolism; Conserved Sequence; Crystallography, X-Ray; Hydrolysis; Mannose/chemistry; Mannose/genetics*; Mannose/metabolism*; Mannosidases/chemistry*; Mannosidases/genetics; Mannosidases/metabolism*; Mutagenesis, Site-Directed; Protein Binding/genetics; Protein Engineering/methods*; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity;

ISSN/eISSN

0006-2960 -

WoS ID

WOS:000278452300020