Zeitschriftenaufsatz
|
2018
An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
Autor:in
de Almeida Monteiro Melo Ferraz, Marcia; Rho, Hoon; Hemerich, Daiane; Henning, Heiko; van Tol, H.; Holker, Michael; Besenfelder, Urban; Mokry, Michal; Vos, P. L. A. M.; Stout, Tom A. E.; Le Gac, Severine; Gadella, Bart
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Journal
Abstrakt
Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi) genome in preparation for the reacquisition of (epi) genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi) genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and preimplantation development, which could improve the quality and (epi) genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development.
Schlagwörter
Animals; Cattle; Cells, Cultured; Cellular Reprogramminggenetics; Epigenesis, Genetic; Female; Fertilization in Vitroinstrumentationmethods; Gene Expression Profiling; Gene Ontology; Genomicsmethods; Humans; Oviductscytologymetabolism; Pregnancy; Zygotegrowth & developmentmetabolism
Dokumententyp
Originalarbeit
CC Lizenz
CCBY
Open Access Type
Gold
ISSN/eISSN
2041-1723 -
10.1038/s41467-018-07119-8
WoS ID
PubMed ID