Immunophenotype of Peripheral Blood Lymphocytes in Dogs with Inflammatory Bowel Disease

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Hirt, Reinhard; Luckschander-Zeller, Nicole; Schwendenwein, Ilse; Richter, Barbara; Rütgen, Barbara; Saalmüller, Armin

Abstrakt

Background: Inflammatory bowel disease (IBD) is common in dogs. Despite the known importance of intestinal lymphocytes in its pathogenesis, little is known about the role of peripheral blood lymphocytes (PBLs) in IBD. Objectives: The aims of this study were (1) comparison of PBLs analyzed by flow cytometry (FCM) in IBD dogs and healthy controls and (2) comparison of PBLs in IBD dogs at the time of diagnosis and in dogs in clinical remission. Animals: Whole blood samples of 19 IBD dogs at the time of diagnosis and blood samples of 6 dogs in clinical remission were collected. Ten healthy dogs served as controls. Methods: In this prospective observational study, PBLs were analyzed with multicolor FCM by staining with a panel of anticanine and cross-reactive monoclonal antibodies against T-and B-cell differentiation antigens, including CD45, CD3, CD4, CD8 alpha, CD8 beta, TCR alpha beta, TCR gamma delta, CD79 alpha cy, and CD21. Results: The IBD patients' PBLs had significantly decreased percentages of TCR gamma delta(+) T lymphocytes (median: healthy dogs, 3.32; IBD dogs, 0.97; P = 0.03) and CD21(+) B cells (median: healthy dogs, 27.61; IBD dogs, 17.26; P = 0.04). There were no significant differences in PBLs between pretreatment and follow-up samples. Conclusions and Clinical Importance: The differences between PBLs in healthy and IBD dogs analyzed by FCM indicate an imbalance of lymphocytes with different immunologic functions and emphasize the potential value of this technique in a larger cohort of dogs. The PBLs did not differ between IBD dogs before treatment and clinically well-controlled dogs after treatment.

Dokumententyp

Originalarbeit

Open Access Type

Gold

DOI

10.1111/jvim.14812

PubMed ID

MEDLINE:28862348