Zeitschriftenaufsatz
|
2017
Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus
Autor:in
Koop, Gerrit; Vrieling, Manouk; Storisteanu, Daniel Matthew L.; Lok, Laurence; Monie, Tom; van Wigcheren, Glenn; Raisen, Claire; Ba, Xiaoliang; Gleadall, Nicholas; Hadjirin, N.; Timmerman, Arjen; Wagenaar, Jaap A.; KLUNDER, H; Fitzgerald, J. Ross; Zadoks, Ruth N.; Paterson, Gavin; Torres, Carmen; Waller, Andrew S.; Loeffler, Anette; Loncaric, Igor; Hoet, Armando; Bergstrom, Karin; De Martino, Luisa; Pomba, Constanca; de Lencastre, Herminia; Ben Slama, Karim; Gharsa, Haythem; Richardson, Emily; Chilvers, E. R.; de Haas, Carla J.C.; van Kessel, Kok; van Strijp, Jos; Harrison, Ewan; Holmes, Mark
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Journal
Abstrakt
Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S-and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (Phi Saeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.
Schlagwörter
Animals; Bacterial Toxinsgeneticsmetabolism; Cattle; Cell Survival; Gene Order; Horse Diseasesmicrobiology; Horses; Host Specificity; Humans; Leukocidinsgeneticsmetabolism; Neutrophilsmetabolism; Phylogeny; Protein Binding; Receptors, Interleukin-8Bmetabolism; Staphylococcal Infectionsmicrobiology; Staphylococcus aureusgeneticsmetabolism
Dokumententyp
Originalarbeit
CC Lizenz
CCBY
Open Access Type
Gold
ISSN/eISSN
2045-2322 -
10.1038/srep40660
WoS ID
PubMed ID