Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Schlötterer, Christian; Nolte, Viola; Barghi, Neda

Abstrakt

The combination of experimental evolution with high-throughput sequencing of pooled individualsi.e., evolve and resequence (E&R)is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following approximate to 60 generations in a novel hot environment for D. melanogaster and D. simulans. For D. simulans, the regions carrying putatively selected loci were far more distinct, and thus harbored fewer false positives, than those in D. melanogaster. We propose that species without segregating inversions and higher recombination rates, such as D. simulans, are better suited for E&R studies that aim to characterize the genetic variants underlying the adaptive response.

Dokumententyp

Originalarbeit

Open Access Type

Gold

DOI

10.1534/g3.117.043349

PubMed ID

MEDLINE:28546383