Zeitschriftenaufsatz | 2019 Open Access

A novel magnet-based scratch method for standardisation of wound-healing assays

Autor:in

Fenu, Michele; Bettermann, T.; Vogl, C.; Darwish, Nadim; Schramel, Johannes Peter; Jenner, Florien; Ribitsch, Iris

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Jenner, Florien; Gerner, Iris; Schramel, Johannes; Vogl, Claus

Journal

Scientific Reports

Abstrakt

A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories.

Schlagwörter

Animals; Biological Assaystandards; Cells, Cultured; Chondrocytescytology; Horses; Magnetic Phenomena; Reference Standards; Wound Healing

Dokumententyp

Originalarbeit

CC Lizenz

CCBY

Open Access Type

Gold

ISSN/eISSN

2045-2322 -

DOI

10.1038/s41598-019-48930-7

WoS ID

WOS:000483697800007

PubMed ID

MEDLINE:31477739

Abstrakt