Corynebacterium urogenitale sp. nov. isolated from the genital tract of a cow

A Gram- stain-positive bacterial isolate, designated LMM-1652(T), was isolated from an intrauterine cytobrush sample originat-ing from a postpartum Holstein Friesian dairy cow. The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative. 16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05% similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %). Subsequent whole genome analysis showed that the genome- to-genome distance of strain LMM-1652(T) to its closest relatives was in the range of 23.2-24.8 %, while the averagenucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species. Strain LMM-1652(T) was characterized by a quinone system mainly consisting of MK-9(H2) and MK-10(H-2). The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group. Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, beta-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found. The cell wall contained meso- diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan. The fatty acid profile was mainly composed of C-18: 1 omega 9c, C-18: 0 and C-16:0. We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652(T) (=LMG 31163(T)=DSM 108747(T)).

Univ Bielefeld
Veterinärmedizinische Universität Wien
University of Bielefeld