Fine Mapping without Phenotyping: Identification of Selection Targets in Secondary Evolve and Resequence Experiments

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Schlötterer, Christian; Dolezal, Marlies

Abstrakt

Evolve and Resequence (E&R) studies investigate the genomic selection response of populations in an Experimental Evolution setup. Despite thepopularity of E&R, empirical studies in sexually reproducingorganismstypically suffer from an excess of candidate locidue to linkagedisequilibrium, and single geneor SNPresolutionis the exception rather than the rule. Recently, so-called "secondary E&R" has beensuggestedas promising experimental follow-upprocedure toconfirmputatively selected regions fromaprimary E&R study. Secondary E&R provides also the opportunity to increase mapping resolution by allowing for additional recombination events, which separate the selection target from neutral hitchhikers. Here, we use computer simulations to assess the effect of different crossing schemes, population size, experimental duration, and number of replicates on the power and resolution of secondary E&R. We find that the crossing scheme and population size are crucial factors determining power and resolution of secondary E&R: A simple crossing schemewith fewfounder lines consistently outcompetes crossing schemeswhere evolved populations froma primary E&R experiment aremixedwith a complex ancestral founder population. Regardless of the experimental design tested, a population size of at least 4,800 individuals, which is roughly five times larger than population sizes in typical E&R studies, is required to achieve a power of at least 75%. Our studyprovides an important tep toward improved experimental designs aiming to characterize causative SNPs in Experimental Evolution studies.

Dokumententyp

Originalarbeit

Open Access Type

Gold

DOI

10.1093/gbe/evab154

PubMed ID

MEDLINE:34190980