Zeitschriftenaufsatz | 2022 Open Access

A novel enterovirus in lambs with poliomyelitis and brain stem encephalitis

Autor:in

Weissenboeck, Herbert; Ebinger, Arnt; Gager, Anna; Thaller, D.; Hoeper, Dirk; Lichtmannsperger, Katharina; Weissenbacher-Lang, Christiane; Matt, J.; Beer, Martin

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Weissenböck, Herbert; Thaller, Denise; Lichtmannsperger, Katharina; Matt, Julia; Weissenbacher-Lang, Christiane

Journal

Transboundary and Emerging Diseases

Abstrakt

An Austrian organic dairy sheep farm experienced cases of recumbency and sudden deaths in 3- to 4-week-old lambs. Two animals were subjected to thorough clinical and pathological investigations. Pathohistological analysis identified severe nonsuppurative myelitis and mild nonsuppurative encephalitis. A reverse-transcription quantitative PCR (RT-qPCR) assay for the recently discovered ovine picornavirus causing comparable lesions scored negative. By next-generation sequencing-based metagenomics, a nearly complete genome of a novel enterovirus could be detected and assembled. In situ hybridization using a specifically designed probe revealed robust signals in affected motoneurons of the spinal cord suggesting a causative role of the novel virus.

Schlagwörter

encephalomyelitis; enterovirus; in situ hybridization; lamb; next-generation sequencing

Dokumententyp

Originalarbeit

CC Lizenz

CCBY

Open Access Type

Hybrid

ISSN/eISSN

1865-1674 - 1865-1682

DOI

10.1111/tbed.14412

WoS ID

WOS:000732468800001

PubMed ID

MEDLINE:34874614

Abstrakt