Serological detection of antibodies to Coxiella burnetii, Chlamydia abortus, Toxoplasma gondii and Leptospira spp. in New World camelids from Austria

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Schmoll, Friedrich

Abstrakt

Introduction New World camelids are susceptible to the infectious agents Coxiella burnetii, Chlamydia abortus, Toxoplasma gondii and Leptospira spp., which cause reproductive disorders and pose a risk of human infection (zoonosis). We have investigated the presence of these infectious agents in New World camelids from Austria. Materials and Methods We tested blood sera taken from Austrian New World camelids in the years 2018, 2019 and 2020 for the presence of antibodies to Coxiella burnetii (619 sera from 536 animals), Chlamydia abortus (619 sera from 536 animals), Toxoplasma gondii (711 sera from 628 animals) and Leptospira spp. (711 sera from 628 animals). Two samples from 41 animals and three samples from 21 animals taken on different dates were available. We used ELISA to detect antibodies to Coxiella burnetii, Chlamydia abortus and Toxoplasma gondii (ID Screen (R) Q Fever Indirect Multispecies, ID Screen (R) Chlamydophila abortus Indirect Multispecies and ID Screen (R) Toxoplasmosis Indirect Multispecies, IDVet, France) and a microscopic agglutination test for antibodies against Leptospira spp. (serovars Australis, Canicola, Copenhageni, Grippothyposa, Hardjo, Pomona, Saxkoebing and Sejroe). A sample was considered positive for Leptospira antibodies if it tested positive in at least one serovar (titer >= 1 :100). Results We found the following results (positive/questionable/negative): Coxiella burnetii (1/1/617), Clamydia abortus (0/1/618), Toxoplasma gondii (276/12/423) and Leptospira spp. (51/4524). In subsequent samples, the first Coxiella burnetii antibody-positive animal was negative, while 18 Toxoplasma gondii antibody-negative animals were positive, two Leptospira antibody-negative animals were positive and four Leptospira antibody-positive animals were negative or showed a decreased antibody titre. Conclusion New World camelids from Austria have varying levels of antibodies against Coxiella burnetii, Chlamydia abortus, Toxoplasma gondii and Leptospira spp. The pathogens should thus be considered as a differential diagnosis to protect the health of animals and humans.

Dokumententyp

Originalarbeit

Open Access Type

Diamond

DOI

10.5680/wtm000006