Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere

Autor:in

Kampfer, Peter; Glaeser, Stefanie; Busse, Hans-Juergen; McInroy, John A.; Clermont, Dominique; Criscuolo, Alexis

Abstrakt

A facultative anaerobic, Gram-stain-positive, endospore-forming bacterium, isolated from the rhizosphere of maize roots (Zea mays), was taxonomically studied. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-79(T) clustered only loosely with Neobacillus species and showed the highest similarity to Neobacillus soli (97.9%). The 16S rRNA gene sequence similarities to the sequences of the type strains of other Neobacillus species were 97.5% and below. Chemotaxonomic features supported the grouping of the strain to the Neobacillus group, e.g. the major fatty acids were C 15:0 anteiso, C 15:0 iso and C 16:0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid, the major quinone was menaquinone MK-7, and major compound in the polyamine pattern was spermidine. However, the JJ-79(T) genome assembly did not share most of the 11 conserved signature indels that are indicative of the genus Neobacillus. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-79(T) genome assembly and those of the closest relative Bacillaceae type strains were <71, <71and <25%, respectively. Physiological and biochemical test results were also different from those of the most closely related Bacillaceae species. As a consequence, JJ-79(T) represents a novel genus for which we propose the name Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., with JJ-79(T) (=CIP 111885(T)=CCM 9045(T)) as the type strain.

Dokumententyp

Originalarbeit

DOI

10.1099/ijsem.0.005367

PubMed ID

MEDLINE:35550233