Sigla storax (Liquidambar orientalis) mitigates in vitro methane production without disturbances in rumen microbiota and nutrient fermentation in comparison to monensin

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Khiaosa-Ard, Ratchaneewan; Gruber, Theresa; Chizzola, Remigius; Zebeli, Qendrim

Abstrakt

Aim The aim of this study was to investigate the in vitro dose-dependent effects of sigla storax (Styrax liquidus) on rumen microbiota and rumen microbial fermentation in comparison to monensin as a positive control. Methods and Results This study was carried out using a rumen simulation model (Rusitec). Treatments consisted of no additive (control), 10 mg l(-1) of monensin sodium salt, 100 mg l(-1) (Low-Sigla), and 500 mg l(-1) (High-Sigla) of sigla storax (n = 6/treatment). In addition to rumen fermentation characteristics, rumen microbial composition was investigated using 16S rRNA sequencing. The methane variables and the acetate to propionate ratio decreased in the both High-Sigla and monensin groups (P < 0.05). High-Sigla had no effect on ammonia, total SCFA and nutrition degradation, while monensin decreased these parameters (P < 0.05). Unlike monensin, the sigla storax treatments did not affect the alpha or beta diversity indexes of the microbiota. The relative abundance of Methanomethylophilaceae and Ruminococcaceae decreased with High-Sigla and monensin (P < 0.05), and Atopobiaceae and Eggerthellaceae decreased with the both doses of sigla storax as well as monensin treatments (P < 0.05). Syntrophococcus, DNF00809, and Kandleria were among the genera that most decreased with High-Sigla and monensin (Q < 0.07) and were strongly positively correlated with methane production (r = 0.52-0.56). Conclusions The high dose of sigla storax (500 mg l(-1)) decreased methane in the rumen ecosystem without adverse effects on nutrient degradation and SCFA production, and without dramatically impacting the microbial composition. Sigla storax might be a novel feed additive to mitigate methane in cattle.

Dokumententyp

Originalarbeit

DOI

10.1093/jambio/lxad154

PubMed ID

MEDLINE:37475660