RNA sample optimization for cryo-EM analysis

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Glatt, Sebastian

Abstrakt

RNAs play critical roles in most biological processes. Although the three-dimensional (3D) structures of RNAs primarily determine their functions, it remains challenging to experimentally determine these 3D structures due to their conformational heterogeneity and intrinsic dynamics. Cryogenic electron microscopy (cryo-EM) has recently played an emerging role in resolving dynamic conformational changes and understanding structure-function relationships of RNAs including ribozymes, riboswitches and bacterial and viral noncoding RNAs. A variety of methods and pipelines have been developed to facilitate cryo-EM structure determination of challenging RNA targets with small molecular weights at subnanometer to near-atomic resolutions. While a wide range of conditions have been used to prepare RNAs for cryo-EM analysis, correlations between the variables in these conditions and cryo-EM visualizations and reconstructions remain underexplored, which continue to hinder optimizations of RNA samples for high-resolution cryo-EM structure determination. Here we present a protocol that describes rigorous screenings and iterative optimizations of RNA preparation conditions that facilitate cryo-EM structure determination, supplemented by cryo-EM data processing pipelines that resolve RNA dynamics and conformational changes and RNA modeling algorithms that generate atomic coordinates based on moderate- to high-resolution cryo-EM density maps. The current protocol is designed for users with basic skills and experience in RNA biochemistry, cryo-EM and RNA modeling. The expected time to carry out this protocol may range from 3 days to more than 3 weeks, depending on the many variables described in the protocol. For particularly challenging RNA targets, this protocol could also serve as a starting point for further optimizations. This protocol describes an iterative workflow to optimize RNA preparation conditions for cryo-EM analysis. Cryo-EM data processing to resolve RNA conformational changes and modeling into cryo-EM density are also described.Existing protocols have not extensively described the variables in RNA sample preparation, cryo-EM data processing and modeling, thus are difficult to standardize. Our iterative approach is generally applicable to RNAs and can serve as a starting point for challenging RNAs. This protocol describes the optimization of RNA preparation conditions for cryo-EM structure determination, along with cryo-EM processing pipelines to resolve RNA dynamics and conformational changes, and workflows to generate moderate- to high-resolution cryo-EM density maps.

Dokumententyp

Übersichtsarbeit

DOI

10.1038/s41596-024-01072-1

PubMed ID

MEDLINE:39548288