Identification of CD4⁺ T cells with T follicular helper cell characteristics in the pig

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Mair, Kerstin; Grebien, Florian; Eder, Thomas

Abstrakt

T follicular helper (Tfh) cells provide help to germinal center B cells for affinity maturation, class switch and memory formation. Despite these important functions, this subset has not been studied in detail in pigs due to a lack of species-specific antibodies. We investigated putative Tfh cells from lymphoid tissues and blood of healthy pigs by using cross-reactive antibodies for inducible T-cell costimulator (ICOS) and B-cell lymphoma 6 (Bcl-6). In lymph nodes, we identified a CD4(+) T cell population with an ICOS(+)Bcl-6(+)CD8 alpha(+) phenotype, reminiscent of human and murine germinal center Tfh cells. Within blood-derived CD4(+) T cells, sorted ICOSh- iCD25(-) and ICOS(dim)CD25(dim) cells were able to induce the differentiation of CD21(+)IgM(+) B cells into Ig-secreting plasmablasts. Compared to naive CD4(+) T cells, these two phenotypes were 3-to 7-fold enriched for cells expressing the Tfh-related transcripts CD28, CD40LG, IL6R and MAF, as identified by single-cell RNA sequencing. These results provide a first characterization of Tfh cells in swine and confirm their ability to provide B-cell help.

Dokumententyp

Originalarbeit

Open Access Type

Hybrid

DOI

10.1016/j.dci.2022.104462

PubMed ID

MEDLINE:35667468