Zeitschriftenaufsatz | 2025

Determining the effects of pseudouridine incorporation on human tRNAs

Autor:in

Biela, Anna; Nowak, Jakub; Biela, Artur; Mukherjee, Sunandan; Moafinejad, Seyed Naeim; Maiti, Satyabrata; Chramiec-Glabik, Andrzej; Mehta, Rahul; Jezowski, Jakub; Dobosz, Dominika; Dahate, Priyanka; Arluison, Veronique; Wien, Frank; Indyka, Paulina; Rawski, Michal; Bujnicki, Janusz M.; Lin, Ting-Yu; Glatt, Sebastian

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Glatt, Sebastian

Journal

EMBO Journal

Abstrakt

Transfer RNAs (tRNAs) are ubiquitous non-coding RNA molecules required to translate mRNA-encoded sequence information into nascent polypeptide chains. Their relatively small size and heterogenous patterns of their RNA modifications have impeded the systematic structural characterization of individual tRNAs. Here, we use single-particle cryo-EM to determine the structures of four human tRNAs before and after incorporation of pseudouridines (Psi). Following post-transcriptional modifications by distinct combinations of human pseudouridine synthases, we find that tRNAs become stabilized and undergo specific local structural changes. We establish interactions between the D- and T-arms as the key linchpin in the tertiary structure of tRNAs. Our structures of human tRNAs highlight the vast potential of cryo-EM combined with biophysical measurements and computational simulations for structure-function analyses of tRNAs and other small, folded RNA domains.

Schlagwörter

tRNA; Cryo-EM; RNA Modifications; RNA Folding; Molecular Dynamics Simulation

Dokumententyp

Originalarbeit

CC Lizenz

CCBY

Open Access Type

Gold

ISSN/eISSN

0261-4189 - 1460-2075

DOI

10.1038/s44318-025-00443-y