Increasing Complexity of the N-Glycome During Caenorhabditis Development

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Yan, Shi

Abstrakt

Caenorhabditis elegans is a frequently employed genetic model organism and has been the object of a wide range of developmental, genetic, proteomic, and glycomic studies. Here, using an off-line MALDI-TOF-MS approach, we have analyzed the N-glycans of mixed embryos and liquid-or plate-grown L4 larvae. Of the over 200 different annotatable N-glycan structures, variations between the stages as well as the mode of cultivation were observed. While the embryonal N-glycome appears less complicated overall, the liquid-and plate-grown larvae differ especially in terms of methylation of bisecting fucose, alpha-gal-actosylation of mannose, and di-6-galactosylation of core alpha 1,6-fucose. Furthermore, we analyzed the O-glycans by LC-electrospray ionization-MS following 6-elimination; especially the embryonal O-glycomes included a set of phosphorylcholine-modified structures, previously not shown to exist in nematodes. However, the set of glycan structures cannot be clearly correlated with levels of gly-cosyltransferase transcripts in developmental RNA-Seq datasets, but there is an indication for coordinated expression of clusters of potential glycosylation-relevant genes. Thus, there are still questions to be answered in terms of how and why a simple nematode synthesizes such a diverse glycome.

Dokumententyp

Originalarbeit

Open Access Type

Gold

WoS ID

WOS:001003074300001

Repository Phaidra

o:2232