Zeitschriftenaufsatz | 2017 Open Access

O-GlcNAcylation of STAT5 controls tyrosine phosphorylation and oncogenic transcription in STAT5-dependent malignancies

Autor:in
Freund, Patricia; Kerenyi, M. A.; Hager, M.; Wagner, Tobias; Wingelhofer, B.; Pham, Ha Thi Thanh; Elabd, Mohamed; Han, Xiaonan; Valent, Peter; Gouilleux, Fabrice; Sexl, Veronika; Kraemer, Oliver; Groner, Bernd; Moriggl, Richard
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Sexl, Veronika; Freund, Patricia
Journal
Leukemia
Schlagwörter
Acetylglucosaminemetabolism; Animals; Cell Line; Cell Transformation, Neoplastic; Female; Gene Expression Regulation, Neoplastic; Genes, Reporter; Glycosylation; Humans; Interleukin-3pharmacology; Lymphoid Tissuecytology; Male; Mice; Mutagenesis, Site-Directed; Myeloproliferative Disordersetiologygenetics; Phosphorylation; Phosphotyrosinemetabolism; Protein Processing, Post-Translational; Radiation Chimera; Recombinant Fusion Proteinsmetabolism; STAT5 Transcription Factorgeneticsmetabolism; Signal Transduction; T-Lymphocytescytologydrug effectsmetabolism; Threoninemetabolism; Transcriptional Activation; Tumor Suppressor Proteinsgeneticsmetabolism
Dokumenten­typ
Originalarbeit
CC Lizenz
CC-BY
Open Access Type
Hybrid
ISSN/eISSN
0887-6924 - 1476-5551
DOI
Öffnen URL 10.1038/leu.2017.4
WoS ID
Öffnen URL WOS:000412179600014
PubMed ID
Öffnen URL MEDLINE:28074064

Weitere Details

Band
31
Startseite
2132
Letzte Seite
2142
Nummer
10
Seiten­anzahl
11
The signal transducer and activator of transcription 5 (STAT5) regulates differentiation, survival, proliferation and transformation of hematopoietic cells. Upon cytokine stimulation, STAT5 tyrosine phosphorylation (pYSTAT5) is transient, while in diverse neoplastic cells persistent overexpression and enhanced pYSTAT5 are frequently found. Post-translational modifications might contribute to enhanced STAT5 activation in the context of transformation, but the strength and duration of pYSTAT5 are incompletely understood. We found that O-GlcNAcylation and tyrosine phosphorylation act together to trigger pYSTAT5 levels and oncogenic transcription in neoplastic cells. The expression of a mutated hyperactive gain-of-function (GOF) STAT5 without O-GlcNAcylation resulted in decreased tyrosine phosphorylation, oligomerization and transactivation potential and complete loss of oncogenic transformation capacity. The lack of O-GlcNAcylation diminished phospho-ERK and phospho-AKT levels. Our data show that O-GlcNAcylation of STAT5 is an important process that contributes to oncogenic transcription through enhanced STAT5 tyrosine phosphorylation and oligomerization driving myeloid transformation. O-GlcNAcylation of STAT5 could be required for nutrient sensing and metabolism of cancer cells.

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