Zeitschriftenaufsatz
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2016
A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model
Autor:in
Diesner-Treiber, Susanne C.; Bergmayr, Cornelia; Pfitzner, Barbara; Assmann, Vera; Krishnamurthy, Durga Durga; Starkl, Philipp; Endesfelder, David; Rothballer, Michael; Welzl, Gerhard; Rattei, Thomas; Eiwegger, Thomas; Szepfalusi, Zsolt; Fehrenbach, Heinz; Jensen-Jarolim, Erika; Hartmann, Anton; Pali-Schoell, Isabella; Untersmayr, Eva
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Journal
Abstrakt
In our mouse model, gastric acid-suppression is associated with antigen-specific IgE and anaphylaxis development. We repeatedly observed non-responder animals protected from food allergy. Here, we aimed to analyse reasons for this protection. Ten out of 64 mice, subjected to oral ovalbumin (OVA) immunizations under gastric acid-suppression, were non-responders without OVA-specific IgE or IgG1 elevation, indicating protection from allergy. In these non-responders, allergen challenges confirmed reduced antigen uptake and lack of anaphylactic symptoms, while in allergic mice high levels of mouse mast-cell protease-1 and a body temperature reduction, indicative for anaphylaxis, were determined. Upon OVA stimulation, significantly lower IL-4, IL-5,1L-10 and IL-13 levels were detected in non-responders, while IL-22 was significantly higher. Comparison of fecal microbiota revealed differences of bacterial communities on single bacterial Operational-Taxonomic-Unit level between the groups, indicating protection from food allergy being associated with a distinct microbiota composition in a non-responding phenotype in this mouse model. (C) 2016 The Authors. Published by Elsevier Inc.
Schlagwörter
Food allergy; Allergen uptake; Intestinal barrier function; Cytokines; Microbiota; Bacterial community composition0
Dokumententyp
Originalarbeit
CC Lizenz
CCBY
Open Access Type
Hybrid
ISSN/eISSN
1521-6616 - 1521-7035
10.1016/j.clim.2016.10.009
WoS ID
PubMed ID