Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Walzer, Christian; Loncaric, Igor; Grünzweil, Olivia Mariel; Szostak, Michael; Spergser, Joachim

Abstrakt

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum beta-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene bla(CMY) (n = 51) was the predominant beta-lactamase gene. In addition, bla(TEM-1) (n = 38), bla(SHV-33) (n = 8), bla(CTX-M-15) (n = 7), bla(OXA-1) (n = 7), bla(SHV-11) (n = 3), and bla(DHA-1) (n = 2) were detected. The most prevalent non-beta-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.

Dokumententyp

Originalarbeit

Open Access Type

Gold

WoS ID

WOS:000660227300001