Detection of Extended Spectrum ß-Lactamase-Producing Escherichia coli with Biofilm Formation from Chicken Meat in Istanbul

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Paulsen, Peter

Abstrakt

Antimicrobial resistance is one of the major public health problems worldwide. This study aimed to detect the presence of extended-spectrum beta-lactamase-(ESBL-)producing Escherichia (E.) coli in chicken meat in Istanbul, T & uuml;rkiye. Raw chicken meat samples (n = 208) were collected from different sale points and analyzed for ESBL-producing E. coli. In total, 101 (48.5%) isolates were confirmed as E. coli by PCR, of which 80/101 (79.2%) demonstrated multiple antibiotic resistance. Resistance against amoxicillin-clavulanic acid was most frequent (87.1%). Eighteen isolates (17.8%) demonstrated phenotypical ESBL resistance, as assessed by the double disc synergy test (DDST). Isolates were tested for the presence of beta-lactamase genes and mobilized colistin-resistant genes. The bla(TEM) group was most frequently detected (97.02%), followed by bla(CTX m) (45.5%), bla(SHV) (9.9%), and bla(OXA-2) (0.9%). However, mcr genes and bla(NDM,) bla(KPC), bla(VIM), and bla(OXA-48) genes were not found in any isolate. E. coli strains were tested for biofilm formation in six different media [Nutrient broth, LB broth, Tryptone Soya broth (TSB), TSB containing 1% sucrose, TSB containing 0.6% yeast extract, and BHI]. Biofilm formation by E. coli isolates (44/101, 43.5%) was highest in TSB with 1% sucrose. It is worth noting that all biofilm-producing isolates were found to harbor the bla(TEM-1) gene, which can indicate a high level of antibiotic resistance. This is the first report about ESBL-producing E. coli in poultry meat, the exposure of consumers in Istanbul metropolitan areas, and the ability of E. coli from this region to produce biofilms.

Dokumententyp

Originalarbeit

Open Access Type

Gold

WoS ID

WOS:001201672900001

Repository Phaidra

o:3620