Diversity of Staphylococcus aureus isolated from nares of ruminants

Aims To examine the diversity of Staphylococcus aureus isolated from nasal swabs of ruminants in Rwanda.Methods and results A total of 454 nasal swabs from 203 cows, 170 goats, and 81 sheep were examined for the presence of S. aureus, and 30 S. aureus isolates were detected and characterized pheno- and genotypically. Resistance to penicillin and/or tetracycline was observed. The isolates were assigned to eight different spa types (t21057 (novel), t10103, t18853, t20842, t318, t355, t458, and t9432) belonging to six clonal complexes (CCs) (CC152, CC30, CC3591, CC3666, CC522, and CC97). Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83), and the human and bovine variants of the toxic shock syndrome toxin gene tst-1 variants were detected.Conclusion These findings demonstrate that the nares of ruminants in Rwanda are colonized with mastitis-associated S. aureus, including lineages that are also carried by humans, underscoring the zoonotic risk, especially for livestock keepers. These results highlight the crucial importance of hygiene measures when handling livestock. To examine the diversity of Staphylococcus aureus isolated from nasal swabs of ruminants in Rwanda.A total of 454 nasal swabs from 203 cows, 170 goats, and 81 sheep were examined for the presence of S. aureus, and 30 S. aureus isolates were detected and characterized pheno- and genotypically. Resistance to penicillin and/or tetracycline was observed. The isolates were assigned to eight different spa types (t21057 (novel), t10103, t18853, t20842, t318, t355, t458, and t9432) belonging to six clonal complexes (CCs) (CC152, CC30, CC3591, CC3666, CC522, and CC97). Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83), and the human and bovine variants of the toxic shock syndrome toxin gene tst-1 variants were detected.These findings demonstrate that the nares of ruminants in Rwanda are colonized with mastitis-associated S. aureus, including lineages that are also carried by humans, underscoring the zoonotic risk, especially for livestock keepers. These results highlight the crucial importance of hygiene measures when handling livestock.© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.

Leibniz Inst Photon Technol IPHT
InfectoGnost Res Campus
Dresden Univ Hosp
New Vision Veterinary Hospital
University of Rwanda
Complexity Science Hub Vienna CSH - Verein zur Förderung wissenschaftlicher Forschung im Bereich komplexer Systeme
Veterinärmedizinische Universität Wien
Österreichische Agentur für Gesundheit und Ernährungssicherheit
New Vis Vet Hosp NVVH Northern Prov
Leibniz Institut fur Photonische Technologien
Technische Universitat Dresden
Friedrich Schiller University of Jena
Free University of Berlin
Freie Universität Berlin
Friedrich-Schiller-Universität Jena