Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach

Publikationen als Autor:in / Herausgeber:in der Vetmeduni

Pratscher, Barbara; Csukovich, Georg; Brandt, Sabine; Burgener, Iwan; Freund, Patricia

Abstrakt

Given the high prevalence of intestinal disease in humans and animals, there is a strong need for clinically relevant models recapitulating gastrointestinal systems, ideally replacing in vivo models in accordance with the principles of the 3R. We established a canine organoid system and analysed the neutralising effects of recombinant versus natural antibodies on Clostridioides difficile toxins A and B in this in vitro system. Sulforhodamine B cytotoxicity assays in 2D and FITC-dextran barrier integrity assays on basal-out and apical-out organoids revealed that recombinant, but not natural antibodies, effectively neutralised C. difficile toxins. Our findings emphasise that canine intestinal organoids can be used to test different components and suggest that they can be further refined to also mirror complex interactions between the intestinal epithelium and other cells.

Dokumententyp

Originalarbeit

Open Access Type

Gold

DOI

10.3390/ijms24043867

PubMed ID

MEDLINE:36835278

Projekt

Establishment of canine intestinal organoids as alternative model for the assessment of biologics and drugs; Identification of cell populations in canine intestinal organoids and primary epithelium for modelling of acute and chronic gastrointestinal diseases.