Zeitschriftenaufsatz | 2023 Open Access

Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach

Autor:in
Csukovich, Georg; Kramer, Nina; Pratscher, Barbara; Gotic, Ivana; Freund, Patricia; Hahn, Rainer; Himmler, G.; Brandt, Sabine; Burgener, Iwan A.
Publikationen als Autor:in / Herausgeber:in der Vetmeduni
Pratscher, Barbara; Csukovich, Georg; Brandt, Sabine; Burgener, Iwan; Freund, Patricia
Journal
International Journal of Molecular Sciences
Schlagwörter
organoids; Clostridioides difficile; sIgA; neutralisation; toxin
Dokumenten­typ
Originalarbeit
CC Lizenz
CC-BY
Open Access Type
Gold
ISSN/eISSN
1661-6596 - 1422-0067
DOI
Öffnen URL 10.3390/ijms24043867
WoS ID
Öffnen URL WOS:000939218200001
PubMed ID
Öffnen URL MEDLINE:36835278
Repository Phaidra
Öffnen URL o:1377
Pro­jekt
Establishment of canine intestinal organoids as alternative model for the assessment of biologics and drugs; Identification of cell populations in canine intestinal organoids and primary epithelium for modelling of acute and chronic gastrointestinal diseases.

Weitere Details

Band
24
Nummer
4
Seiten­anzahl
15
Given the high prevalence of intestinal disease in humans and animals, there is a strong need for clinically relevant models recapitulating gastrointestinal systems, ideally replacing in vivo models in accordance with the principles of the 3R. We established a canine organoid system and analysed the neutralising effects of recombinant versus natural antibodies on Clostridioides difficile toxins A and B in this in vitro system. Sulforhodamine B cytotoxicity assays in 2D and FITC-dextran barrier integrity assays on basal-out and apical-out organoids revealed that recombinant, but not natural antibodies, effectively neutralised C. difficile toxins. Our findings emphasise that canine intestinal organoids can be used to test different components and suggest that they can be further refined to also mirror complex interactions between the intestinal epithelium and other cells. Given the high prevalence of intestinal disease in humans and animals, there is a strong need for clinically relevant models recapitulating gastrointestinal systems, ideally replacing in vivo models in accordance with the principles of the 3R. We established a canine organoid system and analysed the neutralising effects of recombinant versus natural antibodies on Clostridioides difficile toxins A and B in this in vitro system. Sulforhodamine B cytotoxicity assays in 2D and FITC-dextran barrier integrity assays on basal-out and apical-out organoids revealed that recombinant, but not natural antibodies, effectively neutralised C. difficile toxins. Our findings emphasise that canine intestinal organoids can be used to test different components and suggest that they can be further refined to also mirror complex interactions between the intestinal epithelium and other cells.


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